Person: ÇELİK, ÖZGE
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ÇELİK
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ÖZGE
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Publication Metadata only Tuza Toleranslı Tütün Mutantlarında Yaprak Tüylerinin Ultrastrüktürel İncelenmesi(2011-10-25) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653; 4019Publication Metadata only An investigation on regulation on trichome metabolism related genes against salt stress in soybean (Glycine max L. Merr.)(2014-06-25) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653; 4019Publication Embargo Response of soybean plants to gamma radiation: Biochemical analyses and expression patterns of trichome development(2014) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 6653; 113987; 4019This is the first report on soybean with the aim to show the effects of gamma radiation on trichome metabolim. Soybean seeds were subjected to 300 Gy gamma radiation at a dose rate of 10 Gy/min using a Cs-137 gamma source. The photosynthetic pigment, total protein content and ascorbate peroxidase activity were studied. The results showed that the chlorophyll a content was decreased by 80% on day 14 and by 77% on day 21 of irradiation. The chlorophyll b content was reduced by 58.6% and 62.06% on day 14 and 21 after irradiation, respectively. The total carotenoid concentration was reduced by 81.14% on the 14th day after irradiation and by 91% on the 21st day of irradiation, compared to control. The total protein concentration was found to have decreased significantly at 14 and 21 day after treatment. High level of ascorbate peroxidase (APX) activity was recorded in the leaves developed from irradiated soybean seeds, compared to the non-irradiated group. The trichome densities were 6.76 fold increased at 21 day of irradiation, while the stomatal densities were decreased, compared to control. We also performed a qRT-PCR analysis to detect the transcription levels of the soybean trichome developmental genes. The GL2 and CPC genes were up-regulated (P≤0.05). The results of this study pointed out that the CPC transcription factor has to be study in further studies to provide an insight on its exact role in regulation of trichome development in soybean under radiation stress.Publication Metadata only Gama Radyasyonun Soya (Glycine max (L.) Merrill) Yapraklarının Ultrastrüktürel ve Biyokimyasal Özellikleri Üzerine Etkisi(2009-06-22) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653; 4019Publication Metadata only Comparative transcriptional profiling of soybean orthologs of arabidopsis trichome developmental genes under salt stress(Springer, 233 Spring St, New York, Ny 10013 USA, 2018-02) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 6653; 4019The aim of this study was to determine the ultrastructural changes and regulation of trichome-metabolism-related genes against salt stress in soybean (Glycine max L. Merr.) plants. The 14-day-old Ataem-7 and S04-05 soybean seedlings were subjected to 0, 50, 100, and 150 mM NaCl stress. While the chlorophyll quantities were reduced, the activities of guaiacol peroxidase were increased in both varieties due to increasing NaCl concentrations. In S04-05 soybean variety, trichome densities were increased on both surfaces of the leaves whereas decreases were recorded in Ataem-7 variety at 150 mM NaCl treatment. Stomatal densities were increased on both surfaces of the leaves of both soybean varieties after salinity stress. We also performed a qRT-PCR analysis to evaluate the relative transcription levels of the soybean orthologs of Arabidopsis trichome developmental genes. qRT-PCR analysis demonstrated an induction of the soybean orthologs of GL2 and GL3 genes in soybean plants after 50, 100, and 150 mM NaCl treatments in both varieties. While the expression level of TTG1 ortholog gene was negatively affected in both soybean varieties under different concentrations of salinity, GL1 ortholog gene expression profile differed as a result of changing salt concentrations in both varieties with respect to control plants. It is observed that the regulation of trichome formation differs between two soybean varieties.Publication Metadata only Effects of Salinity Stress on Densities of Trichomes, Glandular Trichomes, and Stomata in Two Soybean Varieties(Springer, 233 Spring St, New York, Ny 10013 USA, 2010-09) Suludere, Zekiye; ATAK, ÇİMEN; ÇELİK, ÖZGE; 113987; 4019; 6653