Publication:
Using Raman Spectroscopy to Investigate the Molecular Level Characteristics of Endometriosis

No Thumbnail Available
Date
2020
Authors
Çelik, Sefa
Usta, Abdullah Taner
Özel, Ayşen E.
Yılmaz, Gözde
Yılmaz, Salih
Journal Title
Journal ISSN
Volume Title
Publisher
Pleiades Publishing Inc.
Research Projects
Organizational Units
Journal Issue
Abstract

Endometriosis is a benign gynecologic disorder. It is particularly common among young women and may make pregnancy difficult. In this study molecular level characterization of endometriosis tissues were performed using Raman spectroscopy in combination with multivariate statistical analysis. Three hundred sixty six Raman spectra recorded from different points of seventy two tissue samples, taken from the cyst walls of twelve patients were examined. Principle component analysis (PCA) followed by linear discriminant analysis (LDA) were performed on the Raman data, and the samples were then classified into three groups: severe, moderate, and weak endometriosis. In the severe endometriosis group, the relative band intensities of DNA were increased. Moreover, increase in pyrrole moieties and kynurenine were seen. The results show that endometriosis severity correlates to increase in DNA concentration, and degradation of tryptophan due to increased indoleamine-pyrrole 2,3-dioxygenase (IDO) activity, and an increase in kynurenine concentration and pyrrole intermediate. It is concluded that Raman spectroscopy is capable of providing a quick diagnosis, ahead of the pathology result being reported.

Description
Keywords
Endometriosis , PCA-LDA Analysis , Raman Spectra
Citation
Akyuz, S., Celik, S., Usta, A. T., Ozel, A. E., Yılmaz, G., & Yılmaz, S. (2020). Using Raman Spectroscopy to Investigate the Molecular Level Characteristics of Endometriosis. Optics and Spectroscopy, 128(8), 1318-1327.