Publication: Random amplified polymorphic DNA analysis of salt-tolerant tobacco mutants generated by gamma radiation
Funpec-Editora, Rua Floriano Peixoto 2444, Alto Da Boa Vista, Ribeirao Preto, Sp 00000, Brazil
Salinity is one of the major problems limiting the yield of agricultural products. Radiation mutagenesis is used to improve salt-tolerant mutant plants. In this study, we aimed to improve salt-tolerant mutants of two oriental tobacco varieties. One thousand seeds of each variety (M-0) were irradiated with 100, 200, 300, and 400 Gy gamma rays by Cs-137 gamma. In the M-1 generation, 2999 single plants were harvested. The next season, these seeds were bulked and planted to obtain M-2 progeny. The seeds of 1900 M-2 plants were picked separately. Salinity tolerance was tested in the M-3 generation. Among M-3 plantlets, 10 salt-tolerant tobacco mutants were selected. According to the results of the selection studies, 100- and 200-Gy gamma radiation doses were the effective doses to obtain the desired mutants. Glutathione reductase enzyme activities of salt-tolerant tobacco mutants were determined biochemically as a stress-tolerance marker. The differences between control and salt-tolerant mutants belonging to the Akhisar 97 and Izmir Ozbas tobacco varieties were evaluated by random amplified polymorphic DNA analysis. The total polymorphism rate was 73.91%.
Mutation breeding, Salt tolerance, Glutathione reductase, Random amplified polymorphic DNA