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Polyamine Depletion Enhances The Roscovitine-Induced Apoptosis Through The Activation Of Mitochondria In HCT116 Colon Carcinoma Cells

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Author
Arısan, Elif Damla
Çoker, Ajda
Palavan Ünsal, Narçin
Type
Article
Date
2012-02
Language
en_US
Metadata
Show full item record
Abstract
Small molecule inhibitors of cyclin-dependent kinases (CDKs) show high therapeutic potential in various cancer types which are characterized by the accumulation of transformed cells due to impaired apoptotic machinery. Roscovitine, a CDK inhibitor showed to be a potent apoptotic inducer in several cancer cells. Polyamines, putrescine, spermidine and spermine, are biogenic amines involved in many cellular processes, including apoptosis. In this study, we explored the potential role of polyamines in roscovitine-induced apoptosis in HCT116 colon cancer cells. Roscovitine induced apoptosis by activating mitochondrial pathway caspases and modulating the expression of Bcl-2 family members. Depletion of polyamines by treatment with difluoromethylornithine (DFMO) increased roscovitine-induced apoptosis. Transient silencing of ornithine decarboxylase, polyamine biosynthesis enzyme and special target of DFMO also increased roscovitine-induced apoptosis in HCT116 cells. Interestingly, additional putrescine treatment was found pro-apoptotic due to the presence of non-functional ornithine decarboxylase (ODC). Finally, roscovitine altered polyamine catabolic pathway and led to decrease in putrescine and spermidine levels. Therefore, the metabolic regulation of polyamines may dictate the power of roscovitine induced apoptotic responses in HCT116 colon cancer cells.
Subject
polyamines
apoptosis
roscovitine
DFMO
colon carcinoma
cyclin-dependent kinase
breast-cancer cells
lymphocytic-leukemia cells
r-roscovitine
inhibitor roscovitine
cdk inhibitor
s-phase
b-cll
combination
seliciclib
poliaminler
kolon karsinoması
siklin bağımlı kinaz
göğüs kanseri hücreleri
lenfositik lösemi hücreleri
kombinasyon
URI
http://hdl.handle.net/11413/1602
Collections
  • Makaleler / Articles [140]
  • Pubmed Publications [149]
  • Scopus Publications [724]
  • WoS Publications [1016]

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İstanbul Kültür University

Hakkında |Politika | Kütüphane | İletişim | Send Feedback | Admin

Istanbul Kültür University, Ataköy Campus E5 Karayolu Üzeri Bakırköy 34158, İstanbul / TURKEY
Copyright © İstanbul Kültür University

Creative Commons Lisansı
IKU Institutional Repository, Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Designed by  UNIREPOS