- PublicationOpen AccessAntioxidant enzyme profile in chronic pelvic pain syndrome patients(İstanbul Kültür Üniversitesi Yayınları, 2007-03) Arısan, Elif Damla; Arısan, Serdar; Kiremit, Murat Can; Palavan-Ünsal, Narçin; Ergenekon, ErbilChronic pelvic pain syndrome (CPPS) is a common and serious health problem affecting the quality of life in men. According to National Institute of Health classification system abacterial chronic pelvic pain has two sub category which are defined as 3a and 3b. The major difference between these two groups inflammation status. In this study we aim to investigate the antioxidant enzyme levels such as Glutathion peroxidase (Gsh-Px), superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation products in CPPS patients. All enzyme levels were determined in patients and disease free control group blood samples by spectrophotometrically. In our study, the enzyme levels were not a good indicator for comparison of 3a or 3b type CPPS patients. However, Gsh-Px, SOD and lipid peroxidation products were significantly different from disease free control samples. Although high generation of H202 due to SOD activity, we did not detect any change in CAT activity in patients blood samples compare to control group.
- PublicationOpen AccessSustainability and Regeneration of Ecological Systems in Western Pennsylvania, USA: Research and Efforts(İstanbul Kültür Üniversitesi, 2007-03) Kefeli, V.; Leininger, C.
- PublicationOpen AccessIdentification of clinic uropathogen Escherichia coli isolates by antibiotic susceptibility, plasmid and whole cell protein profiles(İstanbul Kültür Üniversitesi, 2007-03) Çelebi, Ayten; Duran, Nizam; Öztürk, Fatma; Açık, Leyla; Aslan, Gönül; Aslantaş, ÖzkanThe aim of this research was to evaluate the protein, plasmid and antibiotic resistance patterns among 118 uropathogen E. coli strains from infected urinary systems. Plasmids were detected 113 strains (97%). Some isolates harboured up to 10 plasmids, ranging from 1 to 19 kb in size. The total whole cell protein profiles of the strains were obtained using the sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) method. The protein bands were stained with Coomassie-blue and analyzed by statistics package POPGEN. The 118 E. coli were also analyzed for their resistance to antimicrobial agents. The highest rates of resistance were against ampicillin (61 %) and amoxicillin-clavulanic acid (46.6 %). The most common antimicrobial resistance of these isolates was ampicillin, amoxicillin-clavulanic acid, trimethoprimsulfamethoxazole, gentamicin, ciprofloxacin, amikacin, cefoxitin, and ceftriaxone. Multiple resistance to all antibiotics except imipenem was observed in 5 isolates. Similarity matrix and dendrograms were generated by using UPGMA algorithm which made it possible to evaluate the similarity or intra-specific polymorphism degrees based on whole-cell protein fingerprinting, plasmid profiles and antibiotic resistance pattern. It was determined that the SDS-PAGE method may provide better criteria than plasmid and antimicrobial susceptibility for the taxonomic and epidemiological studies of E. coli isolates.
- PublicationOpen AccessSpatial arrangement of the animal male germ cell genome: I. Non-random pattern of radiation-induced inversions involving the vestigial region in autosome 2 of Drosophila melanogaster(İstanbul Kültür Üniversitesi, 2007-03) Alexandrov, Igor; Alexandrova, Margarita V.; Korablinova, Svetlana V.; Korovina, Larisa N.At present, given a bulk of models for higher-order chromatin structure in interphase nuclei of animal somatic cells, little is known about the spatial chromosome organization in animal male germ cells mainly due to the lack suitable methods for detailed observation and analysis without disruption of the existing organization. We pioneered in the study of this issue via analysis of radiation-induced inversion patterns in Drosophila male germ cell genome taking into account the fact that the formation of inversion requires the spatial proximity and contact of its ends. Analysis of 72 γ- ray- or neutron-induced vg inversions in which the first break is invariantly associated with the vestigial (vg) gene in the middle of 2R autosome shows the second inversion breakpoints that highly non-randomly distributed over the entire second chromosome clustering at the three autosome 2 “hot” chromosome areas. These findings show that the polar Rabl-configuration of interphase chromosomes in various somatic cells is not typical for the haploid genome of Drosophila mature sperms. The specific megarosetteloopstructure of haploid chromosome in the mature animal sperms is proposed and justified.
- PublicationOpen AccessResveratrol: Chemoprevention with red wine(İstanbul Kültür Üniversitesi, 2007-03) Arısan, Elif Damla; Palavan-Ünsal, NarçinAccording to epidemiological studies, western diet has disadvantages because of cancer prevalence more than Mediterranean or Asia people who consume more vegetables and fruits. Resveratrol (trans-3,4,5-trihydroxystilbene) which is highly found in grapes, berries has received attention for its potential chemopreventive and antitumor effects in experimental systems. Because of high resveratrol content, researchers noted that red wine has multidimensional benefits for cardiovascular health. Resveratrol also protects neuron cells from other well known chemotherapeutics. The molecular function of resveratrol in chemoprevention and carcinogenesis are reviewed by experimental cancer cell models. Resveratrol is going to be a promising molecule in future cancer prevention and therapy models.