Show simple item record

dc.contributor.advisorAlkurt, Gizem
dc.contributor.authorÖzfiliz Kılbaş, Pelin
dc.contributor.authorKızılboğa, Tuğba
dc.contributor.authorDemir, Salih
dc.contributor.authorPalavan Ünsal, Zeynep Narçın
dc.contributor.authorArısan, Elif Damla
dc.contributor.authorDinler Doğanay, Gizem
dc.date.accessioned2018-07-13T07:16:35Z
dc.date.available2018-07-13T07:16:35Z
dc.date.issued2015-07
dc.identifier.issn0263-6484
dc.identifier.other1099-0844
dc.identifier.urihttps://doi.org/10.1002/cbf.3114
dc.identifier.urihttps://hdl.handle.net/11413/2065
dc.description.abstractBag-1, Bcl-2 associated athanogene-1, is a multifunctional protein that can regulate a wide variety of cellular processes: proliferation, cell survival, transcription, apoptosis and motility. Bag-1 interacts with various targets in the modulation of these pathways; yet molecular details of Bag-1's involvement in each cellular event are still unclear. We first showed that forced Bag-1 expression promotes cell survival and prevents drug-induced apoptosis in MCF-7 breast cancer cells. Increased mRNA expressions of c-myc protooncogene and ornithine decarboxylase (ODC), biosynthetic enzyme of polyamines, were detected in Bag-1L+ cells, and western blots against the protein product of c-Myc and ODC confirmed these findings. Once ODC, a c-Myc target, gets activated, polyamine biosynthesis increases. We observed enhanced polyamine content in the Bag-1L+ cells. On the contrary, when polyamine catabolic mechanisms were investigated, Bag-1 silencing suppressed biosynthesis of polyamines because of the downregulation of ODC and upregulation of PAO. Exposure of cells to apoptotic inducers enhances the cell death mechanism by producing toxic products such as H2O2 and aldehydes. Bag-1L+ cells prevented drug-induced PAO activation leading to a decrease in H2O2 production following cisplatin or paclitaxel treatment. In this line, our results suggested that Bag-1 indirectly affects cell survival through c-Myc activated signalling that causes elevation of ODC levels, leading to an increase of the polyamine content. Copyright (c) 2015 John Wiley & Sons, Ltd.tr_TR
dc.language.isoen_UStr_TR
dc.publisherWiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ USAtr_TR
dc.relationCell Biochemistry and Functiontr_TR
dc.subjectBag-1tr_TR
dc.subjectc-Myctr_TR
dc.subjectPolyaminestr_TR
dc.subjectPaclitaxeltr_TR
dc.subjectCisplatintr_TR
dc.subjectApoptosistr_TR
dc.subjectBreast cancertr_TR
dc.titleBag-1 promotes cell survival through c-Myc-mediated ODC upregulation that is not preferred under apoptotic stimuli in MCF-7 cellstr_TR
dc.typeArticletr_TR
dc.contributor.authorID195744tr_TR
dc.contributor.authorID6125tr_TR
dc.contributor.authorID113920tr_TR
dc.contributor.authorID152975tr_TR
dc.identifier.wos358622200005
dc.identifier.wos358622200005en
dc.identifier.scopus2-s2.0-84937776194
dc.identifier.scopus2-s2.0-84937776194en
dc.identifier.pubmed26178413
dc.identifier.pubmed26178413en


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record