• Home
  • About
  • Policies
  • Contact
    • Türkçe
    • English
  • English 
    • Türkçe
    • English
  • Login
Advanced Search
View Item 
  •   Home
  • Fen Edebiyat Fakültesi / Faculty of Letters and Sciences
  • Moleküler Biyoloji ve Genetik / Molecular Biology and Genetics
  • Makaleler / Articles
  • View Item
  •   Home
  • Fen Edebiyat Fakültesi / Faculty of Letters and Sciences
  • Moleküler Biyoloji ve Genetik / Molecular Biology and Genetics
  • Makaleler / Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

CDK inhibitors-induced SSAT expression requires NF kappa B and PPAR gamma in MCF-7 breast cancer cells

Thumbnail
View/Open
CDK inhibitors-induced SSAT expression requires NF kappa B and PPAR gamma in MCF-7 breast cancer cells.pdf (997.2Kb)
Author
Obakan Yerlikaya, Pınar
Yıldırım, Şeyma
Öztürk, Mert Burak
Berrak, Özge
Çoker Gürkan, Ajda
Arısan, Elif Damla
Ünsal Palavan, Zeynep Narçın
Type
Article
Date
2015
Language
en_US
Metadata
Show full item record
Abstract
The cyclin-dependent kinase (CDK) inhibitors purvalanol and roscovitine are therapeutic agents that control cell proliferation through regulating cell-cycle machinery. They also affect polyamine (PA) metabolism, which is activated in malignant tissues. Therefore, PA catabolism became a remarkable target in cancer therapies. Induction of the PA catabolic enzyme spermidine/spermine N-1-acetyltransferase (SSAT) is under the control of transcription factors such as NF kappa B and PPAR gamma. The purpose of this study was to investigate the therapeutic potential of CDK inhibitors in combination with PAs in MCF-7 breast cancer cells. In order to understand the involvement of PA catabolic enzyme SSAT in this process we also checked its transcriptional regulation in the presence of CDK inhibitors. MCF-7 cells were exposed to CDK inhibitors in the absence or presence of Spd and Spm. Cell viability loss was evaluated by MTT assay. Apoptosis was determined by annexin-V/PI staining using FACS flow. The SSAT transcription level was measured by qRT-PCR. Intracellular PA pool was determined by HPLC. Protein expressions were assessed by western blotting. We found that CDK inhibitors decreased cell viability in a time-dependent manner and induced apoptosis. Co-treatment of Spd or Spm with CDK inhibitors prevented the apoptotic potential of both drugs. Purvalanol increased SSAT expression levels in a time-dependent manner. Although the induction of SSAT by purvalanol resulted in the activation of NF kappa B at early time points, induction was accomplished by PPAR gamma as a late response after purvalanol treatment. We concluded that both transcriptional control mechanisms could be responsible for SSAT regulation in a time-dependent manner.
Subject
Polyamines
SSAT
Purvalanol
PPAR gamma
NF kappa B
Spermidine/Spermine N-1-Acetyltransferase SSAT
Induced Apoptosis
Colon-Cancer
Polyamine Metabolism
Carcinoma Cells
Activation
Induction
Roscovitine
Autophagy
Alpha
URI
https://doi.org/10.3906/biy-1501-18
https://hdl.handle.net/11413/2130
Collections
  • Makaleler / Articles [140]
  • WoS Publications [1016]

İstanbul Kültür University

Hakkında |Politika | Kütüphane | İletişim | Send Feedback | Admin

Istanbul Kültür University, Ataköy Campus E5 Karayolu Üzeri Bakırköy 34158, İstanbul / TURKEY
Copyright © İstanbul Kültür University

Creative Commons Lisansı
IKU Institutional Repository, Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Designed by  UNIREPOS

İKU Kütüphane


Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypeLanguageBy PublisherRightsPubmedScopusWoSThis CollectionBy Issue DateAuthorsTitlesSubjectsTypeLanguageBy PublisherRightsPubmedScopusWoS

My Account

Login

İstanbul Kültür University

Hakkında |Politika | Kütüphane | İletişim | Send Feedback | Admin

Istanbul Kültür University, Ataköy Campus E5 Karayolu Üzeri Bakırköy 34158, İstanbul / TURKEY
Copyright © İstanbul Kültür University

Creative Commons Lisansı
IKU Institutional Repository, Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Designed by  UNIREPOS