Publication: Genetic Similarity between Ottoman Strawberry and the Other Early-Period Strawberry Cultivars Assessed by RAPD Markers
Date
2017
Authors
Çelik, Özge
Doğan, H.
Akdaş, E. Y.
Uslu, Y. B.
Zengin, U.
Journal Title
Journal ISSN
Volume Title
Publisher
Austin Publishing Group
Abstract
Most of the local strawberry (Fragaria x ananassa Duchenese) cultivars
of Turkey are not phylogenetically classified. This present study, which aimed
discriminating the genetical relationships between the Ottoman strawberry
cultivar and other early-period strawberry cultivars planted in Istanbul and
Karadeniz Ereğli regions of Turkey, is the first on this field. Seven strawberry
cultivars were analyzed by using RAPD. 10 random primers carried out DNA
fingerprinting analyses of these cultivars. The average polymorphism rate was
determined as 87.1%. The most polymorphic primer was OPH01 which produced
13 bands. The cluster dendrogram presented that the similarity coefficients
were between range of 0.03 and 0.73. Ottoman cultivar showed higher similarity
with Tüylü more than Kara cultivar which is reported as the mother cultivar.
RAPD method was sufficient to assess the phylogenetic relationship between
Ottoman cultivar and other early-period strawberry cultivars. The data of this
study brought forward the necessity of further genetic analysis to prove the
phylogenetic relationship among Tüylü and Ottoman cultivars.
Description
Keywords
RAPD, genetic similarity, Ottoman strawberry cultivar, Early-period strawberry