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Roscovitine treatment inhibited cell proliferation and induced apoptosis via modulating activity of Stat3 and Foxo1 under control of Akt in LNCaP prostate cancer cells

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Roscovitine treatment inhibited cell proliferation and induced apoptosis via modulating activity of Stat3 and Foxo1 under control of Akt in LNCaP prostate cancer cells.pdf (235.8Kb)
Author
Arısan, Elif Damla
Gümüşkaptan, Çağrı
Berrak, Özge
Obakan Yerlikaya, Pınar
Çoker Gürkan, Ajda
Ünsal, Zeynep Narçin
Type
conferenceObject
Date
2015
Language
en_US
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Abstract
Cyclin dependent kinases (CDKs) are known activators of nuclear hormone receptors; such as androgen receptor (AR) via modulating several cell survival signaling routes. However, novel CDK inhibitors might also prevent cell proliferation and induce apoptosis in different prostate cancer cells regardless of functional AR status. Purine-derived purvalanol and roscovitine are strong apoptotic inducers, caused cell cycle arrest through competing ATP binding sites of several CDKs and disrupt cyclin/CDK complex formation. However, their mechanistic action in prostate cancer cells is not fully understood. In this study, we found that, exposure of LNCaP and DU145 prostate cancer cells to purvalanol (20 mM) and roscovitine (30 mM) for 24 h induced apoptosis compared to untreated prostate cancer cells. According to immunoblotting results, both CDK inhibitors downregulated Stat 3 and caused dephosphorylation of Stat3 in cytoplasmic lysates. In addition, we found that although roscovitine was less effective on Stat3-Foxo1 proteins interaction due to upstream Akt phosphorylation compared to purvalanol treated samples, Stat3-AR binding affinity was decreased in LNCaP cells. Therefore we concluded that while roscovitine were modulating Akt phosphorylation and altered Foxo nuclear transport, it also affected Stat expression profile to induce cell cycle arrest which caused cell death in prostate cancer cells.
URI
https://doi.org/10.4172/1948-5956.S1.044
https://hdl.handle.net/11413/4415
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  • Bildiriler, Kongreler ve Sempozyumlar / Declarations, Congresses and Symposiums [103]

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Hakkında |Politika | Kütüphane | İletişim | Send Feedback | Admin

Istanbul Kültür University, Ataköy Campus E5 Karayolu Üzeri Bakırköy 34158, İstanbul / TURKEY
Copyright © İstanbul Kültür University

Creative Commons Lisansı
IKU Institutional Repository, Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Designed by  UNIREPOS