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dc.contributorFen Edebiyat Fakültesi / Faculty of Letters and Sciences Moleküler Biyoloji ve Genetik / Molecular Biology and Geneticstr_TR
dc.contributor.authorEralp, Tuğçe Nur
dc.contributor.authorArısan, Elif Damla
dc.contributor.authorÖzfiliz, Pelin
dc.contributor.authorObakan Yerlikaya, Pınar
dc.contributor.authorÇoker Gürkan, Ajda
dc.contributor.authorDinler Doğanay, Gizem
dc.contributor.authorÜnsal, Zeynep Narçin
dc.date.accessioned2019-02-13T08:41:15Z
dc.date.available2019-02-13T08:41:15Z
dc.date.issued2014-11
dc.identifier.issn1513-7368
dc.identifier.other2476-762X
dc.identifier.urihttp://dx.doi.org/10.7314/APJCP.2014.15.11.4475
dc.identifier.urihttps://hdl.handle.net/11413/4548
dc.description.abstractBackground: Cisplatin, a DNA damaging agent, induces apoptosis through increasing DNA fragmentation. However, identification of intrinsic resistance molecules against Cisplatin is vital to estimate the success of therapy. Bag-1 (Bcl-2-associated anthanogene) is one anti-apoptotic protein involved in drug resistance impacting on therapeutic efficiency. Elevated levels of this protein are related with increase cell proliferation rates, motility and also cancer development. For this reason, we aimed to understand the role of Bag-1 expression in Cisplatininduced apoptosis in HeLa cervix cancer cells. Cisplatin decreased cell viability in time- and dose-dependent manner in wt and Bag-1L+HeLa cells. Although, 10μM Cisplatin treatment induced cell death within 24h by activating caspases in wt cells, Bag-1L stable transfection protected cells against Cisplatin treatment. To assess the potential protective role of Bag-1, we first checked the expression profile of interacting anti-apoptotic partners of Bag-1. We found that forced Bag-1L expression prevented Cisplatin-induced apoptosis through acting on Mcl-1 expression, which was reduced after Cisplatin treatment in wt HeLa cells. This mechanism was also supported by the regulation of heat shock protein (Hsp) family members, Hsp90 and Hsp40, which were involved in the regulation Bag-1 interactome including several anti-apoptotic Bcl-2 family members and c-Raf.tr_TR
dc.language.isoen_UStr_TR
dc.relationAsian Pacific Journal of Cancer Preventiontr_TR
dc.subjectBag-1tr_TR
dc.subjectHsp familytr_TR
dc.subjectcervical cancertr_TR
dc.subjectapoptosistr_TR
dc.subjectdrug resistancetr_TR
dc.titleBag-1L mediated chemoresistance mechanism through preventing downregulation of Mcl-1 and c-Raf by heat shock proteins in HeLa cellstr_TR
dc.typeArticletr_TR
dc.contributor.authorID272026tr_TR
dc.contributor.authorID113920tr_TR
dc.contributor.authorID195744tr_TR
dc.contributor.authorID156421tr_TR
dc.contributor.authorID125860tr_TR
dc.contributor.authorID152975tr_TR
dc.contributor.authorID6125tr_TR


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